Which Technique Should I use for Trace Metal Analysis?

Trace Metal Analysis

Trace Metal Analysis

Estimation of trace metals in different studies such as environmental samples, drinking water, pharmaceuticals, foods and beverages, minerals, geological samples, clinical samples and forensic samples are assuming greater importance due to increased awareness on both the nutritional values and harmful effects of trace metals.

Before going into the techno-commercial considerations it is necessary to go into the merits and limitations of common available options:

Gravimetric Analysis

Gravimetric analysis is based on estimation of elemental composition of the isolated compound by precipitation, purification and final ignition to constant weight. It is useful when a high degree of sensitivity is needed. It is a very time consuming technique but without high investment cost. The main sources of error in gravimetric analysis are co-precipitation and solubility of precipitates

Volumetric Titrations

Volumetric determinations are less time consuming in comparison to gravimetric estimations.. The accuracy of determinations is dependent upon correct volumetric readings and observation of endpoint colour changes. Electrometric endpoint detection titrations generally overcome this source of error.

Spectroscopic Methods

Spectroscopic methods are mostly based on development of coloured complexes of the trace metals with ligand groups on the reagents. Spectroscopic estimations consume much less time in comparison with the gravimetric and volumetric determinations but the accuracy of results is dependent on various factors such as pH of the solutions, selection of absorption wavelength, temperature variations and inherent errors in absorbance readings.

Atomic Absorption Spectroscopy

Atomic Absorption Spectroscopy is by far the most common and affordable technique used for analysis of trace metals. It affords estimations ranging upto ppm levels using flame technique and ppb levels for graphite furnace or hydride generation techniques. The limiting factor is that only one element can be analysed at a time and for multielement detection a different light source is required and on an average about 3 min are required for each element determination.

ICP Spectroscopic Techniques

ICP techniques afford simultaneous detection of a number of elements in a sample in the same time as it would take to analyse a single element in AAS. Both ICP – OES and ICP – MS techniques are versatile but ICP – MS in addition provides isotopic ratio of same element present in the sample. Both ICP – OES and ICP – MS are costly techniques requiring greater operational costs as well. However, both the techniques provide sub – ppb level determinations and very large sample throughputs. Due to these advantages it is possible to recover the cost on initial investment over a short period of time

As you can see several options are available for trace metal estimations and you have to choose from the available options based on your analysis requirements. The choice of the estimation technique is ultimately governed by a number of factors such as:

  • Cost of the instrument
  • Cost of analysis in terms of the reagent costs and cost of operating utilities such as gases
  • Number of samples to be analysed
  • Required limits of detection and concentration range over which determinations are to be made
  • Availability of sample amount

Hopefully you will like the coverage of the article. Please share your experiences and offer your valuable comments.

About Dr. Deepak Bhanot

Dr Deepak Bhanot is a seasoned professional having nearly 30 years expertise beginning from sales and product support of analytical instruments. After completing his graduation and post graduation from Delhi University and IIT Delhi he went on to Loughborough University of Technology, UK for doctorate research in analytical chemistry. His mission is to develop training programs on analytical techniques and share his experiences with broad spectrum of users ranging from professionals engaged in analytical development and research as well as young enthusiasts fresh from academics who wish to embark upon a career in analytical industry.

Comments

  1. Kundo Hundang says:

    Dear Dr.Deepak Bhanot,
    Is there any opportunity to do a PhD in Analytical chemistry and instrumentation in India under your Supervison. I would like to find out more if such opportunity exists. Thankyou

    • Dear Kundo,
      You can forward me your details of education,experience,your field of interest and any topic of research that you have in mind on my e-mail. After review I shall offer my suggestions and comments.

  2. dear sir
    in addition to the above methods, X-ray spectroscopy is more frequently being used in jewellary testing, ayurvedic bhasmas, mineral identification, geology, metallurgy which needs to be included. Interpretation needs expertise
    with regards

    • Thanks Dr Aitha,
      Thanks for the suggestion.Scope for trace metals analysis is indeed vast and there are some areas which have not been covered. We also welcome guest articles. In case you have expertise in this area you can also send us a brief article which after review will be published under your name on the site.

  3. Sir I am daily gaining knowledge through your articles,
    Thanks
    Ajay

  4. Nureni Bashiru says:

    You are really a source of inspiration to me sir, Keep it up sir.

  5. mahamad atef youssef saladin says:

    i think for me a more information can be afforded here

  6. I FOUND IT VERY INTERASTING .Again request U To Post Me More On This Topic

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