The reliability of any HPLC analysis depends to a great extent on precautions and considerations taken on nature of the sample and characteristic properties of component solvents of the mobile phase.In other words the mobile phase is the lifeline of HPLC system and its compatibility with the sample and column packing material is a major consideration before taking up any analysis.
Key Considerations in preparation and use of HPLC Mobile Phase
Dr Deepak Bhanot is a seasoned professional having nearly 30 years expertise beginning from sales and product support of analytical instruments. After completing his graduation and post graduation from Delhi University and IIT Delhi he went on to Loughborough University of Technology, UK for doctorate research in analytical chemistry. His mission is to develop training programs on analytical techniques and share his experiences with broad spectrum of users ranging from professionals engaged in analytical development and research as well as young enthusiasts fresh from academics who wish to embark upon a career in analytical industry.