Concept of Theoretical Plates in Column Chromatography

As a chromatographer you would be interested in getting the maximum return from your column in terms of performance efficiency. Useful tips on care of HPLC columns have been suggested earlier to preserve the performance features and improve useful life of your column. Now the question arises as to how do you quantify efficiency and decide […]

The role of Stationary Phase particle size on HPLC separations

Column length and particle size of stationary phase play a crucial role in separation efficiency and the column back pressure of HPLC columns. Longer columns and smaller particle size contribute to improved separation efficiency but at cost of increased column backpressure. Classification of HPLC separations by column dimensions covers the classification of HPLC separations into […]

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