How to prevent damage to Capillary GC columns

Capillary GC columns are capable of producing highly reproducible chromatograms provided right operational conditions are maintained and steps are taken from time to time to prevent damage and performance degradation. The factors which can result in deterioration are discussed in the present article and preventive measures are suggested: Accidental breakage High-temperature damage Oxygen damage Chemical […]

Benefits of Automated replacement of GC liners

The injector liners tend to get contaminated over a period of time due to injection of dirty samples. Due to adsorption on liner walls there is loss of some sample components and as a result there is a drop in sensitivity accompanied with broadening of the chromatographic peaks. The degree of deterioration of signals depends on […]

Types of Liners and their Selection

A GC inlet liner plays an important role in transfer of the injected sample into the GC column. It provides an inert surface for conversion of the injected liquid sample to vapour and transfer to the column without any change in its characteristics. Liners are available in different design configurations with or without deactivation and […]

Sample Injection Techniques for Capillary Column Gas Chromatography

Whenever you inject a sample into a Gas Chromatograph you are dealing with small micro- volumes of liquids which can be mixtures with differing volatilities in terms of their boiling points and other chemical characteristics. The desirable feature of the sample injection technique is reproducibility of injection volume injection after injection without loss of any […]

Minimization of influence of random fluctuations in operating conditions in GC analysis

More than often you set your operating conditions to your satisfaction but still face unexpected problems during analysis which cannot be reasoned and diagnosed easily. Such unexpected behaviour is quite frustrating and you have to spend considerable amount of your valuable time to iron out such abnormal variations in your analysis. In this article some […]

Why very long capillary GC columns are not preferred?

It is a well-known fact that the separation efficiency of a chromatographic column increases with an increase in its length. Doubling the column length increases resolution by around 40%. Normally capillary GC columns range in length from 15 m to about 60 m. Such column lengths are generally sufficient to resolve complex compounds having closely […]

Sample Injection Practices in Gas Chromatography

The accuracy and reproducibility of your Gas Chromatographic analysis will depend to a great extent on the technique adopted for sample injection. Gas chromatography in contrast to liquid chromatography involves analysis of both liquid as well as gaseous samples. This places more stringent requirements on accurate measurement and transfer of injected volumes. Syringe Injection Syringe […]

How Gas Chromatography can contribute to your career prospects?

Today is the age of specialization and over the years you should develop expertise in your chosen profession. It will give you immense satisfaction to be recognized by your colleagues and industry or academic circles as an authority in your area of specialization. Gas chromatography is an established and popular technique used for separation of components […]

How detector characteristics influence Gas Chromatographic response?

A chromatographic detector serves to identify and quantify the sample components as they reach it in a sequence after separation in the chromatographic column. Common chromatographic detectors, their properties and their applications are discussed in an earlier article. Sensitivity versus specificity or selectivity of a detector It is necessary to understand the difference between sensitivity […]

Peak Height or Peak Area? – Which is the right choice for quantitative chromatographic calculations

A typical chromatogram comprises of several peaks varying in size. The height of each peak is in proportion to the amount of the particular component present in the sample mixture injected into the chromatograph. The chromatogram shown above is an ideal one showing symmetrical shaped peaks rising above a stable horizontal baseline. It can be […]

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