Sample Injection Techniques for Capillary Column Gas Chromatography

Whenever you inject a sample into a Gas Chromatograph you are dealing with small micro- volumes of liquids which can be mixtures with differing volatilities in terms of their boiling points and other chemical characteristics. The desirable feature of the sample injection technique is reproducibility of injection volume injection after injection without loss of any […]

Minimization of influence of random fluctuations in operating conditions in GC analysis

More than often you set your operating conditions to your satisfaction but still face unexpected problems during analysis which cannot be reasoned and diagnosed easily. Such unexpected behaviour is quite frustrating and you have to spend considerable amount of your valuable time to iron out such abnormal variations in your analysis. In this article some […]

Why very long capillary GC columns are not preferred?

It is a well-known fact that the separation efficiency of a chromatographic column increases with an increase in its length. Doubling the column length increases resolution by around 40%. Normally capillary GC columns range in length from 15 m to about 60 m. Such column lengths are generally sufficient to resolve complex compounds having closely […]

Sample Injection Practices in Gas Chromatography

The accuracy and reproducibility of your Gas Chromatographic analysis will depend to a great extent on the technique adopted for sample injection. Gas chromatography in contrast to liquid chromatography involves analysis of both liquid as well as gaseous samples. This places more stringent requirements on accurate measurement and transfer of injected volumes. Syringe Injection Syringe […]

How Gas Chromatography can contribute to your career prospects?

Today is the age of specialization and over the years you should develop expertise in your chosen profession. It will give you immense satisfaction to be recognized by your colleagues and industry or academic circles as an authority in your area of specialization. Gas chromatography is an established and popular technique used for separation of components […]

How detector characteristics influence Gas Chromatographic response?

A chromatographic detector serves to identify and quantify the sample components as they reach it in a sequence after separation in the chromatographic column. Common chromatographic detectors, their properties and their applications are discussed in an earlier article. Sensitivity versus specificity or selectivity of a detector It is necessary to understand the difference between sensitivity […]

Peak Height or Peak Area? – Which is the right choice for quantitative chromatographic calculations

A typical chromatogram comprises of several peaks varying in size. The height of each peak is in proportion to the amount of the particular component present in the sample mixture injected into the chromatograph. The chromatogram shown above is an ideal one showing symmetrical shaped peaks rising above a stable horizontal baseline. It can be […]

Importance of Colour Coding for Gas Cylinders and Lines in Laboratories

Gases constitute an essential utility in modern-day laboratories. Examples of gases commonly used are compressed air, zero air, nitrogen, helium, argon, hydrogen, nitrous oxide and acetylene. Operation of laboratory instruments such as Gas Chromatographs, Atomic Absorption spectrometers, ICP’s and Mass spectrometers is unthinkable without provision of required gases. Let us begin by imagining a scene […]

10 Similarities between High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC)

The dissimilarities between HPLC and GC were discussed in the earlier article- How are Gas chromatography (GC) and High Performance liquid chromatography (HPLC) different?. The present article discusses 10 key features which are common to both the techniques. On going through these features you will recollect your basic understanding of chromatography and appreciate the commonality […]

How are Gas Chromatography (GC) and High Performance Liquid Chromatography(HPLC) different?

Gas Chromatography is an ideal technique for separation and identification of gases or volatile liquids having low molecular weights. The basis of separation is selective distribution of components present in gaseous phase on the solid adsorbent inside a column or on a thin liquid layer adsorbed on an inert solid support or on the inside wall […]

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