HPLC stands for High Performance Liquid Chromatography.

Its earlier name was High Pressure Liquid Chromatography because it involved use of liquid mobile phase requiring higher pressures than gases used in Gas Chromatography. The technique has found immense scope of applications in both academic and industrial laboratories requiring identification and quantification of mixtures of organic compounds.

How are analytical scale and preparative scale HPLC different?

HPLC is a versatile analysis tool that affords separation and quantification of large range of chemical compounds. It finds unlimited applications in basic research, pharmaceuticals, foods, polymers and other similar applications. The availability of numerous columns, stationary phases, mobile phases and detectors makes HPLC a high utility facility in modern laboratories. Analytical scale HPLC is […]

Which technique to use – GC or HPLC?

Chromatography has been used as a versatile technique in the laboratory for separation, identification and quantification of components in mixtures of organic compounds. In the earlier stages Chromatography evolved from column chromatography and planar chromatographic separations based on paper chromatography and thin layer chromatography. Advances in instrumentation technology led to introduction of Gas Chromatography(GC) and High […]

Role of HPLC in Cleaning Validation of Laboratory Glassware

HPLC has made significant contributions in detection and quantitative estimation of organic compounds. In pharmaceutical analysis HPLC has been extensively used in assay of the active ingredient as well as related impurities which are present in formulations in very low concentrations. Estimation of related substances was not possible using other conventional analysis techniques. This unique […]

Restoration of HPLC Column Performance

An HPLC column is capable of giving highly reproducible performance run after run for months together. However, it has a finite lifetime and over a period of time its performance begins to deteriorate as evidenced by distortion of the peak shapes and resolution between them, erratic increase in column back pressure and appearance of ghost […]

Important suggestions for generating reliable HPLC results

HPLC has a marked presence in analytical research and quality control laboratories. Important decisions concerning both manufacturing processes as well as publication of research findings are based on decisions which can be arrived at only if high degree of reliability can be placed on the data generated by the HPLC system. HPLC is a multi […]

Time saving tips for the HPLC user

HPLC has become a major analytical tool for basic research and industrial laboratories. Its significance is evident from the number of HPLC units one can find in modern laboratories. In some laboratories complete sections consisting of several HPLC systems are devoted to HPLC analysis .In spite of the large number of systems and dedicated manpower such […]

How to minimize cost on HPLC column replacements?

HPLC has emerged as a popular separation science technique over the last few decades and today it enjoys enviable position in several basic research and industrial laboratories across the globe. It is common to come across several dozens of HPLC systems in large laboratories due to their numerous analysis solution capabilities. It is every chromatographer’s dream […]

Advantages of Filtering HPLC mobile phase solvents

HPLC analysis uses liquids as carriers to transport the injected sample to the column and then to the detector. Such carrier can be an aqueous – organic mixture, organic – organic mixture of a buffer in combination with an organic solvent. It is important to check for complete miscibility of mobile phase solvents before selecting them […]

Potential applications of HPLC in Clinical laboratories

High performance liquid chromatography or HPLC has played a significant role in clinical laboratories for separation and quantitation of bio markers in different body fluids. Ever since the 1970s liquid chromatography, gas chromatography and thin layer chromatography have provided analytical solutions in such laboratories. However, in the past few decades there has been a general shift […]

Contribution of HPLC to Nutritional Labeling of Foods

HPLC or High Performance Liquid Chromatography  has a   marked presence in modern analytical laboratories. The major applications cover basic research and industrial quality control particularly in studies on biomolecules, pharmaceutical manufacturing and new drug development, stability studies on foods and pharmaceuticals and clinical monitoring of body fluids. The present article covers the contributions of HPLC […]

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