HPLC stands for High Performance Liquid Chromatography.

Its earlier name was High Pressure Liquid Chromatography because it involved use of liquid mobile phase requiring higher pressures than gases used in Gas Chromatography. The technique has found immense scope of applications in both academic and industrial laboratories requiring identification and quantification of mixtures of organic compounds.

HPLC – The new age analytical technique!

Analytical chemistry is becoming a popular choice as a career option for students pursuing the discipline of chemistry. The obvious reason is that it applies knowledge of chemistry to gain understanding of chemical properties of materials. It makes valuable contribution in establishing the quality of natural resources and synthetic industrial products for our consumption. The […]

Critical considerations in HPLC solvent selection

Selection of solvents for use as a mobile phase in HPLC analysis is a key component of method development. It is not possible to hava a universal solvent which will meet all applications and more than often a combination of solvents is decided based on the analysis requirements. Selection of suitable solvents is based on […]

Important role of preventive maintenance in smooth functioning of HPLC systems

Preventive maintenance plays a key role in trouble-free operation of laboratory instruments. It is even more critical for instruments that are required to operate round the clock. HPLC is one such instrument which is expected to run continuously in commercial testing laboratories and industries. In pharma and food manufacturing industries quality of products is required […]

How are analytical scale and preparative scale HPLC different?

HPLC is a versatile analysis tool that affords separation and quantification of large range of chemical compounds. It finds unlimited applications in basic research, pharmaceuticals, foods, polymers and other similar applications. The availability of numerous columns, stationary phases, mobile phases and detectors makes HPLC a high utility facility in modern laboratories. Analytical scale HPLC is […]

Which technique to use – GC or HPLC?

Chromatography has been used as a versatile technique in the laboratory for separation, identification and quantification of components in mixtures of organic compounds. In the earlier stages Chromatography evolved from column chromatography and planar chromatographic separations based on paper chromatography and thin layer chromatography. Advances in instrumentation technology led to introduction of Gas Chromatography(GC) and High […]

Role of HPLC in Cleaning Validation of Laboratory Glassware

HPLC has made significant contributions in detection and quantitative estimation of organic compounds. In pharmaceutical analysis HPLC has been extensively used in assay of the active ingredient as well as related impurities which are present in formulations in very low concentrations. Estimation of related substances was not possible using other conventional analysis techniques. This unique […]

Restoration of HPLC Column Performance

An HPLC column is capable of giving highly reproducible performance run after run for months together. However, it has a finite lifetime and over a period of time its performance begins to deteriorate as evidenced by distortion of the peak shapes and resolution between them, erratic increase in column back pressure and appearance of ghost […]

Important suggestions for generating reliable HPLC results

HPLC has a marked presence in analytical research and quality control laboratories. Important decisions concerning both manufacturing processes as well as publication of research findings are based on decisions which can be arrived at only if high degree of reliability can be placed on the data generated by the HPLC system. HPLC is a multi […]

Time saving tips for the HPLC user

HPLC has become a major analytical tool for basic research and industrial laboratories. Its significance is evident from the number of HPLC units one can find in modern laboratories. In some laboratories complete sections consisting of several HPLC systems are devoted to HPLC analysis .In spite of the large number of systems and dedicated manpower such […]

How to minimize cost on HPLC column replacements?

HPLC has emerged as a popular separation science technique over the last few decades and today it enjoys enviable position in several basic research and industrial laboratories across the globe. It is common to come across several dozens of HPLC systems in large laboratories due to their numerous analysis solution capabilities. It is every chromatographer’s dream […]

Get Free and Instant Access to Lifelong Learning!

Lab-Training.Com provides you free ongoing learning on the fundamentals and latest developments in laboratory and analytical sciences. Come be part of our community of 17000 members!

  • Become an expert
  • Tips, Tricks and Troubleshooting
  • Just few minutes a week
  • Latest from the laboratory world
  • Brush up and strengthen your basics
  • Learn new techniques
  • Short and crisp articles