HPLC has made significant contributions in detection and quantitative estimation of organic compounds. In pharmaceutical analysis HPLC has been extensively used in assay of the active ingredient as well as related impurities which are present in formulations in very low concentrations. Estimation of related substances was not possible using other conventional analysis techniques. This unique […]
HPLC stands for High Performance Liquid Chromatography.
Its earlier name was High Pressure Liquid Chromatography because it involved use of liquid mobile phase requiring higher pressures than gases used in Gas Chromatography. The technique has found immense scope of applications in both academic and industrial laboratories requiring identification and quantification of mixtures of organic compounds.
An HPLC column is capable of giving highly reproducible performance run after run for months together. However, it has a finite lifetime and over a period of time its performance begins to deteriorate as evidenced by distortion of the peak shapes and resolution between them, erratic increase in column back pressure and appearance of ghost […]
HPLC has a marked presence in analytical research and quality control laboratories. Important decisions concerning both manufacturing processes as well as publication of research findings are based on decisions which can be arrived at only if high degree of reliability can be placed on the data generated by the HPLC system. HPLC is a multi […]
HPLC has become a major analytical tool for basic research and industrial laboratories. Its significance is evident from the number of HPLC units one can find in modern laboratories. In some laboratories complete sections consisting of several HPLC systems are devoted to HPLC analysis .In spite of the large number of systems and dedicated manpower such […]
HPLC has emerged as a popular separation science technique over the last few decades and today it enjoys enviable position in several basic research and industrial laboratories across the globe. It is common to come across several dozens of HPLC systems in large laboratories due to their numerous analysis solution capabilities. It is every chromatographer’s dream […]
HPLC analysis uses liquids as carriers to transport the injected sample to the column and then to the detector. Such carrier can be an aqueous – organic mixture, organic – organic mixture of a buffer in combination with an organic solvent. It is important to check for complete miscibility of mobile phase solvents before selecting them […]
High performance liquid chromatography or HPLC has played a significant role in clinical laboratories for separation and quantitation of bio markers in different body fluids. Ever since the 1970s liquid chromatography, gas chromatography and thin layer chromatography have provided analytical solutions in such laboratories. However, in the past few decades there has been a general shift […]
HPLC or High Performance Liquid Chromatography has a marked presence in modern analytical laboratories. The major applications cover basic research and industrial quality control particularly in studies on biomolecules, pharmaceutical manufacturing and new drug development, stability studies on foods and pharmaceuticals and clinical monitoring of body fluids. The present article covers the contributions of HPLC […]
The dissimilarities between HPLC and GC were discussed in the earlier article- How are Gas chromatography (GC) and High Performance liquid chromatography (HPLC) different?. The present article discusses 10 key features which are common to both the techniques. On going through these features you will recollect your basic understanding of chromatography and appreciate the commonality […]
Gas Chromatography is an ideal technique for separation and identification of gases or volatile liquids having low molecular weights. The basis of separation is selective distribution of components present in gaseous phase on the solid adsorbent inside a column or on a thin liquid layer adsorbed on an inert solid support or on the inside wall […]