HPLC stands for High Performance Liquid Chromatography.

Its earlier name was High Pressure Liquid Chromatography because it involved use of liquid mobile phase requiring higher pressures than gases used in Gas Chromatography. The technique has found immense scope of applications in both academic and industrial laboratories requiring identification and quantification of mixtures of organic compounds.

Advantages of HPLC over Thin-layer Chromatography (TLC)

The advantages of GC over TLC were discussed in an earlier article. The characteristics that make HPLC popular were also discussed in the article Why is HPLC the most popular technique in analytical laboratories today. HPLC is the successor of TLC and has made significant contributions to the growth of analytical science and its diverse […]

Introduction to High Performance Liquid Chromatography and it’s parts

High Performance Liquid Chromatography (HPLC) is a popular and versatile technique which provides affordable solutions on separation, identification and quantification of constituents of complex organic samples. At first sight the assembly of complex modules and bunch of tubings might scare you. However, on gaining an insight into the role of each component the mystery will […]

What are guard columns and what benefits they offer in HPLC analysis?

The efficient operation of HPLC system is dependent on freedom of mobile phase and sample from chemical impurities or solid suspensions. Precautions in handling and use of HPLC mobile phase discusses measures that should be adopted during preparation and use of mobile phase. Importance of sample filtration discusses benefits of clean sample injections. HPLC column […]

Solutions to First HPLC Program Quiz

The first HPLC quiz was published on the site on 18 June 2014 and I am glad to inform that a number of our subscribers scored over 80% in response to the discount offer and have earned 20% discount on the course fee. I take this opportunity to congratulate all those who qualified for the […]

Why is HPLC the most popular technique in analytical laboratories today?

HPLC is a separation technique which is extensively used to separate, identify and quantify components of mixtures of organic compounds selectively retained on the stationary phase inside the column using liquid as the carrier phase. It has found widespread applications in academic research as well as industrial monitoring and quality control. If you were to […]

HPLC – which modules require regular calibration?

HPLC on first appearance appears to be a complex system comprising of modular boxes and an intricate bunch of tubings though in modern systems most tubings are concealed inside the modules. If you take a careful look the mystery of the modules will start unfolding and you will be able to resolve them into simple […]

Essential precautions in handling and use of HPLC mobile phase

The desirable properties of the HPLC mobile phase were covered in an earlier article. Practices adopted for preparation and handling of the mobile phase are equally important. At this stage extreme care is required to ensure repeatability and accuracy of results. Useful tips are provided in this article that will help you achieve the desired […]

What are the Desirable Properties of HPLC Mobile Phase?

Mobile phase has been rightly termed as the lifeline of the HPLC system. It plays the important role of transport of the sample through the separation column and subsequently to the detector for identification of the separated components. Mobile phase is seldom a single solvent. It consists of combination of water with organic solvents, aqueous […]

HPLC Quiz : Test Your Eligibility for Discount on Certificate Program on HPLC

A unique discount offer is being made to all those who are interested in joining the HPLC certificate program. The free HPLC e-course was developed to help you grasp the HPLC system component modules and upgrade your laboratory skills. We hope that you have benefited from the free e- course. Now it is our turn […]

How to Mix HPLC Mobile Phase Solvents for consistency of results?

A single solvent seldom meets the requirements for separations carried out using HPLC. In most cases two or more solvents are required to be mixed. In isocratic operation the composition is pre- decided and remains unchanged during the analytical run. On the other hand in gradient elution the final solvent composition is a lot different […]

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