Which is better option for quantitative chromatographic calculations – Peak height or Peak area ?

Peak area for quantification by chromatography is preferable to peak height as it takes into consideration peak shape distortions , if any

Familiarize with the Gas Chromatograph

A Gas Chromatograph like any other analytical instrument has evolved from one with several knobs and dials to one having a simple microprocessor based keypad to control the operational parameters. The simplification has resulted in ease of operation and time saving. An understanding of the main component parts will help in maximum utilization of system capabilities.

Key Considerations in preparation and use of HPLC Mobile Phase

The reliability of any HPLC analysis depends to a great extent on precautions and considerations taken on nature of the sample and characteristic properties of component solvents of the mobile phase.In other words the mobile phase is the lifeline of HPLC system and its compatibility with the sample and column packing material is a major […]

Factors deciding choice of Solvents in laboratory

Solvent properties require a clear understanding before making an appropriate selection of a solvent or a mix of solvents for an analytical application in hand.

Performance Verification of UV – Visible Spectrophotometer

A UV –Visible Spectrophotometer is commonly seen in most laboratories. Due to its simplicity, speed, ease of use and non-destructive analysis features it finds applications in several qualitative and quantitative estimations. It is accepted as a workhorse and its performance reliability is generally taken for granted. However, like any other sophisticated laboratory instrument its performance […]

How to save on recurring cost of Certified Reference Materials and Working Standards

Certified Reference Materials cost a fortune in comparison with even high purity reagents as they bear traceability to globally recognized standard bodies and are supplied with a certificate on their composition. A few mg or ml amount can cost as high as several hundred dollars. Laboratories handling large sample loads can incur heavy recurring costs […]

Calibration Plot

A calibration plot is essential for carrying out quantitative analytical determinations. It is possible to carry out a single determination without a calibration plot as in titrimetry or gravimetry. However, when a number of quantitative determinations are required using common instrument analysis methods the variables affecting your analysis get multiplied and it becomes necessary to […]

Cleaning and Validation of Laboratory Glassware

Cleanliness of laboratory glassware before use is of utmost importance. Analysis results can be communicated with any degree of confidence only if the glassware used is free from contamination from previous analysis. Advanced analytical techniques save time through automation of procedures and also provide better reliability of results through minimization of personal errors. However , […]

Importance of Laboratory Safety

 Laboratory Safety should come naturally and become your habit. Remember you are responsible for your own safety as well as safety of others working around you. A laboratory poses potential risks due to exposure to corrosive and toxic materials, flammable solvents, high pressure gases, explosive chemicals and dangerous microbes. A little care and adherence to […]

Double Beam Advantages Over Single Beam Spectrophotometers

A clear understanding on the concept of Double beam and Single beam optics is an absolute must for a spectroscopist. A common misconception is that in a double beam instrument two beams pass simultaneously through the sample cell and provide a sensitivity advantage over single beam systems. For that matter the more the number of beams passing through the sample cell better will be the sensitivity. Today’s Lab Byte session will help dispel such misconceptions. In Double beam design one beam  component passes through the sample cell and the other through the blank solvent taken  in the reference cell. This arrangement helps compensate errors arising due to changes in any of the operational parameters such as power line fluctuations, stray light and solvent absorbance during absorbance observations which contributes to higher accuracy and precision of results.

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