How to select the right cuvette material for UV- VIS absorbance studies?

Common laboratory absorption studies involve liquids – either as pure solvents or solutions of light absorbing compounds in transparent solvents. Such studies are made possible by making use of containers of precise dimensions, usually 10 mm path length, which are transparent to the wavelength of light required for the purpose. Such absorbance cells are also […]

Double Beam Advantages Over Single Beam Spectrophotometers

A clear understanding on the concept of Double beam and Single beam optics is an absolute must for a spectroscopist. A common misconception is that in a double beam instrument two beams pass simultaneously through the sample cell and provide a sensitivity advantage over single beam systems. For that matter the more the number of beams passing through the sample cell better will be the sensitivity. Today’s Lab Byte session will help dispel such misconceptions. In Double beam design one beam  component passes through the sample cell and the other through the blank solvent taken  in the reference cell. This arrangement helps compensate errors arising due to changes in any of the operational parameters such as power line fluctuations, stray light and solvent absorbance during absorbance observations which contributes to higher accuracy and precision of results.

Similarities and Dissimilarities between GC/MS and LC/MS

GC and HPLC are both chromatography separation techniques which are immensely popular with the analytical chemist. Over the past several decades Mass spectroscopy has contributed significantly to the scope of applications of both GC and HPLC. Hyphenated mass spectroscopy techniques have made possible separation and identification of complex mixtures in a matter of minutes which in earlier […]

How LC – MS/MS Scores over LC – MS?

LC – MS/MS offers a range of benefits to the analytical chemist and such features are highlighted in the present article. However, before listing of such benefits you should appreciate the elementary differences between the two techniques. Single quadrupole mass spectrometer A single quadrupole mass spectrometer comprises of a single quadrupole assembly Q1 used for […]

Additional benefits offered by Mass sensitive detectors over other Liquid Chromatography detectors

HPLC is well established as an analytical technique. Why is HPLC the most popular technique in analytical laboratories today? highlights the reasons that justify this claim. The wealth of information made available by commonly used HPLC detectors can be enriched further by mass sensitive detectors. The idea behind writing this article is not to undermine the contributions […]

Mass Discrimination and Analysis in Mass Spectroscopy

Mass Spectroscopy essentially involves ionization of neutral molecules and fragmentation followed by grouping of the ionized species on the basis of their charge to mass (m/z) ratios for detection and quantitation. The present article discusses the common available options for discrimination of the ionized mass fragments and their analysis. Quadrupole Mass Analyzers A quadrupole mass […]

What is the difference between GC – MS Full Scan and Selected Ion Monitoring?

In order to understand the difference between mass spectroscopic full scan and selected ion monitoring it is essential for you to have a clear concept of spectral scanning in optical spectroscopy as both have several commonalities. Scanning in optical spectroscopy Optical spectroscopy serves as a valuable tool for qualitative and quantitative estimation of constituents of […]

Potential Scope of Hyphenated Mass Spectroscopic Techniques

Hyphenated techniques involve physical coupling of instruments through a compatible interface. Hyphenation results in a marked improvement of analysis in terms of speed, specificity and sensitivity. The benefits of coupling are realized mainly in chromatographic separations involving the popular GC and HPLC techniques. Such combinations have received a great deal of attention from analytical chemists for identification […]

Popular Ionization Techniques in Mass Spectroscopy

Ionization plays a central role in mass spectroscopy as it ionizes neutral molecules to charged molecules whose separation in space and time is based on their mass to charge (m/z) ratios. The mass spectroscopist has several available options for initiating ionization depending on analytical requirements and nature of molecules. Common ionization techniques are briefly outlined […]

Essentials of Mass Spectroscopy

Mass spectroscopy is distinctly different from other spectroscopic techniques. It has found large number of applications in several areas of basic research and product development covering pharmaceuticals, foods, environmental pollution monitoring, doping investigation in sports, pesticide residue analysis, biochemistry, clinical investigations and petroleum products analysis. The increasing popularity of mass spectroscopy results from its ability […]

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