Calibration Plot

A calibration plot is essential for carrying out quantitative analytical determinations. It is possible to carry out a single determination without a calibration plot as in titrimetry or gravimetry. However, when a number of quantitative determinations are required using common instrument analysis methods the variables affecting your analysis get multiplied and it becomes necessary to construct a calibration plot over a range of concentrations to accommodate influence of such variables.

Ideally a calibration plot originates at the origin or the intersection point of the recorded parameter axis and the concentration axis and emerges as a straight line with increase in concentration. In simple words it means that when concentration is zero the response is also zero and increases in a mathematical relation with increase in concentration. In real situations, calibration plots show deviations from this ideal behavior after a range of concentrations. Today’s lab bytes covers the important considerations that need to be kept in mind in construction and use of calibration plots.

About Dr. Deepak Bhanot

Dr Deepak Bhanot is a seasoned professional having nearly 30 years expertise beginning from sales and product support of analytical instruments. After completing his graduation and post graduation from Delhi University and IIT Delhi he went on to Loughborough University of Technology, UK for doctorate research in analytical chemistry. His mission is to develop training programs on analytical techniques and share his experiences with broad spectrum of users ranging from professionals engaged in analytical development and research as well as young enthusiasts fresh from academics who wish to embark upon a career in analytical industry.

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