Join Our Certificate Course on HPLC

Learn What Relay Goes Into Running a HPLC and Earn a Certificate!

Why we have made this course?

Over the past eight years that I have been heading the contract services business at our company, my team has grown from just over 30 people to more than 200, couple that, with the healthy attrition rate that we see in our industry, for that matter in any industry, I have literally taken hundreds of interviews to build and maintain a strong, high-performance team.

I have taken interviews and hired people at various stages of their career, right from beginners who are just trying to get a break into the industry, to experienced professionals with decades of experience under their belts.

For the beginners, the fact that they don’t have any experience often makes it exceptionally difficult for them to get their first job, to get that great start, getting the experience they need to further their careers.

“Getting a job and experience required to get it, is like the chicken and the egg situation”

Every organisation, including mine, wants the new employee to be productive from day one. We want our team members to start contributing towards the organisation goals, as soon as possible. And that is why more often than not organisations prefer to hire an experienced person who understands what needs to be done and can start delivering from day one.

As we go through the rigmarole of our formal education from school, to college, to masters and for the really crazy ones, like me, doing a Ph.D. we are bombarded with millions of concepts and facts that often, we feel, have absolutely no relevance to our real-life, I’m still to find out why we were taught the Pythagoras theorem or calculus, and then suddenly we get a job, where we are required to use a whole bunch of those facts and concepts that we might have learnt more than a decade ago.

Interacting with all these people that I have hired and seeing them grow professionally and personally, as part of my team, I have realised what experience really means. I strongly believe that the only difference between an inexperienced beginner and experienced professional is the ability to use what we have learnt, to be able to apply all the fundamental concepts that we have all learnt as part of our formal education to our day-to-day work, to our life.

Experience is the process of getting up to speed, getting the ability to use the basic concepts that we learnt and to be able to use them as naturally as we use the alphabet, or as naturally as talking comes to us.

Now wouldn’t it be great if I knew in school itself, that I’m going to be doing normality and morality, calculations, day in and day out in the analytical lab, once I start with my job? I’m sure I would have paid more attention and would have remembered it by heart. Well this is just one example that comes to mind, there are literally hundreds of things that we have been taught over the years and never know how we would use them in real life.

One way that has been used for a long time by organizations to give the beginners the required experience to start working independently, is to provide induction training, once the join the organisation. Induction training usually comprises of a few weeks to a few months of training, during which the beginners are taught about the organisational structure, all standard procedures related to that function and in the case of laboratory given a lot of hands-on training under the supervision of their seniors. But not all organisations have structured induction training programmes and in most of the organisations, the induction process is totally dependent on on-job training.

This course has been developed from and is based on what we feel are the essential training modules for a beginner joining our HPLC lab. It has been designed to give the beginners a through fundamental understanding of not only running a HPLC system but also of all the associated processes, systems and procedures that we need to be able to understand, use and apply each day to be a productive member of the HPLC lab.

What will you get from this course?

This course is different from any other course, textbook, or even on job training that you will get. Though there definitely is no substitute for practical hands-on experience. This course has been designed to make you far better prepared and to help you make the best of the hands on experience, you will get in the future. In fact, in some ways, the course is even better than practical laboratory training.

It will make sure that when you go into the lab, you will not feel lost and in your mind, you will have complete clarity on what exactly you have to do and what is expected of you. This course will give you the self confidence and belief, which is essential for you to succeed when you go and start working in the lab.

When we were developing the content of the course, my team members who have been working in the lab for many years now actually told me after seeing the content that anyone who knows all that we have captured in this course might even know more than what they have learnt over the past many years working in the lab.

Within this course we have tried to distill the combined knowledge of all our team members and tried to capture all the critical points, the small nitty-grittys that make all the difference when working in the lab.

Much More Than Just Principles of HPLC

The first and perhaps the biggest difference between this course and other courses in the fact that we are not only talking about “High Performance Liquid Chromatography” but have gone further and expanded scope to cover all the small and big things associated with HPLC. We have covered everything from laboratory safety, buying and qualifying the equipment, finding the methods, method validation, weighing practices, sample preparation, dilutions, calculation and interpretation of the results, troubleshooting, good laboratory practice and much more.

More Than 25 Videos

The modules are not only just plain text, but have relevant diagrams and in the entire course, there are more than 25 videos giving you a chance to see exactly how some of the critical steps are performed in the laboratory. This gives you a unique opportunity, even if you are attending a live training in the lab, you would not be able to see the steps more than once, whereas the videos can be played over and over again till the time you fully understand what is being done.

Practical Activities That You Can Perform Even at Home

At the end of most modules, you are presented with a relevant activity, like finding a method of calculating results. This helps you practically implement what you have just learnt in that module, the same way you will once you start working in the lab. As part of the activities we will provide you actual HPLC chromatograms and help you interpret the data and calculate the results.

Excel Sheets for Performing Calculations

In the module on calculations we will give you Excel sheet which you can use to perform calculations using any data. The Excel sheets will come in handy when they start working the lab, they will help you save time and also reduce errors. These are the same Excel sheets that our team members use every day out in our laboratory.

Three Important Standard Operating Procedures

As part of the course we will also share with you three actual standard operating procedure. These are the exact documents which are used by our team members in our lab. The SOPs that you will get are-

  1. Standard Operating Procedure for Operation and Calibration of HPLC
  2. Standard Operating Procedure for Operation and Calibration of Single Pan Weighing Balance.
  3. Standard Operating Procedure for Operation and Calibration of Micro-Pipette

These SOPs will help you understand what standard operating procedures are and what the different parts of an SOP are. So when you will start working in the industry SOPs will not be something new for you, but we come to you naturally. If you try searching for these procedures on the net, you will realize that each of them is priced at over $ 200 each. But we will be providing them to you free of cost, as part of this course.

Access to Exclusive Members Only Discussion Forum

As part of this course, you will also get access to an exclusive member’s forum. This is an area where you can ask any questions or queries you might have on any of the topics covered in the course. Members from our team, including Dr. Saurabh Arora and Dr Deepak Bhanot, as well as other students who are taking the course along with you can then come and answer your queries. So as you go through the course, you won’t be alone, members of our team, as well as other fellow students taking the course will be there to help you and support you.

Sequential Access to the Course Material

The delivery medium is another very unique feature of this course, the course comprises of 20 modules, arranged the sequence in which the steps are practically performed in the lab. This makes it much easier for you to learn and then replicate what you have learnt when you start working in the laboratory. Moreover, we will not overwhelm you by giving you the entire course at once, rather we will release the course content slowly, a couple of modules at a time, for you to understand and digest before the next set of modules become available. You will be given access to the entire course material over a period of two months.

Test at the End of The Course

At the end of the course you will be given a multiple-choice test to evaluate your understanding of the training that has been given to you. This will help you make sure that you are actually learnt all the critical points covered in the course. If you don’t perform well at the first go, you can always go back and read the material again, have another look at the videos and then take another attempt at the test.

Certificate of participation

After clearing all the tests you will also get a certificate of participation in the course. We will send your printed & signed copy of the certificate, along with the table of contents of the course, to you by courier. You shall have access to the course material for a total of six months, this time is more than enough for you to go through the course at your own pace to successfully clear the test and ear the certificate.

Special Discounts on All Our Future Courses

As our way of saying thank you you will be given special discounts on all our future courses on topics like Gas Chromatography, Atomic Absorption Spectroscopy, Inductively Coupled Plasma Mass Spectroscopy and many more courses that are planned in the future.

Course Content

“What really goes into running a HPLC?”

We have answered this question through 18 sequential modules, covering not only the theoretical principles of HPLC but everything you need to be able to do to work in a HPLC lab. The titles and introduction to the 18 modules of the course are given below.

1.     Laboratory Safety – safety always comes first

The first and foremost concern  the minute you enter the lab is  to protect yourself,your colleagues and the laboratory environment from harmful effects of toxic substances and hazardous chemicals. You  must familiarize with hazard signs and use of safety gear .An understanding of basic laboratory operations such as storage and handling of materials and spill management will prove useful in the long run..

2.     Time Management – if you fail to plan you plan to fail

. Before you jump to switch on the HPLC simple time management tools will help you to make the most of your day. By spending just a few minutes each day planning what you are going to do and achieve during the day you can enhance your productivity many folds.

3.     Finding and selecting the right method

The very first thing that you need now is a suitable method for analysing a sample. This could be as simple as picking up a standard testing procedure that has been in use  or you might need to search for a method   and even  develop a new method. In most cases if you do a thorough literature search  you will be able to find a method suitable for your application.

4.     HPLC – understand how it works to make it work for you

A thorough understanding of the basic principles and system modules is essential before you proceed  to  analyze your samples. This will make your work more enjoyable, you will get a better grasp of system operation, applications and you will also be able to resolve the common problems that you may face in day to day operations.

5.     Method Validation – validate if you need to

Your lab may not have a specified method for analysis of required sample, and you might have to spend a lot of time in researching literature for a suitable method or perhaps even develop a new one. The method you have selected should stand upto the requirements of the validation process to give required degree of reliability. It is better to adopt a complex method which is validated rather than a simpler method which does not stand to the rigors of method validation.

6.     Buying, Qualifying and Calibrating the HPLC

Generally the system would be available in your laboratory when you begin your work but in some cases the selected method may require HPLC system having special features and specifications. You have to consider your prime requirements, reputation of the supplier to provide you efficient after sales maintenance and technical support. In industrial and commercial laboratories down time of the systems can often lead to enormous financial loses as well as loss of valuable client business. The procured system has also to satisfy tests commonly referred to as IQ, OQ and PQ. Finally you can ensure the reliability of the instrument only if you follow regularly the calibration schedules.

7.     Sourcing the supplies – Chemicals, Standards, Columns, etc.

You will  require chemicals, reagents, solvents, columns,  etc  Many of these might be available in your lab or you might need to source all of them from a suitable vendor. Many a time, one relies  heavily on the purchase department of the organisation which is often non-technical and might not be able to source right items meeting your requirements. Having the knowledge from where you can source your materials can help you in expediting your work.

8.     Sample Preparation – undoubtedly the most critical step

Sample preparation is  undoubtedly the most critical step in any analysis  as it is usually the largest source of variation and can make or break your result. It can be as simple as dissolving your sample in water or it can be a complex multistep extraction followed by derivatization and dilutions. Understanding the importance of each of the steps of sample preparation is essential to be able to carry out the analysis and attain high degree of accuracy and precision.

9.     Weighing – right every time

Weighing is an activity that all of us think is very easy and simple but it is one of the most critical steps and based on our experience we can say that a lot of mistakes happen during weighing. To properly weigh the samples and reagents you need to be able to select the correct balance, make sure it is calibrated, perform  calibration checks and only then will you be able to get the correct results.

10.    Volumetric Measurements  – vital for dilutions-only practice will make you perfect

The samples which you come across may not be having the desired concentration level of analyte for optimum sensitivity and will often require dilutions. Dilution stages often require several steps and error at any single step gets multiplied in subsequent steps. It is important to use calibrated volumetric glassware as well as good quality calibrated micro pipettes for solution transfer. More important is correct handling of micropipettes so that dilution errors are minimized. Besides sample preparation dilutions are also critical in the preparation of mobile phases , buffers and derivatizing agents, etc.

11.     System suitability

Once the column is conditioned and a stable baseline is achieved it is usual practice to run a few system suitability tests, and if it is a new column that you are using you might have to run column performance tests even before you  start using your system. You need to evaluate the data for parameters like theoretical plate number, peak asymmetry, resolution, etc and only if these parameters meet system suitability requirements  can you proceed with the analysis.

12.     Standards – sourcing, handling, need and traceability

You would need to run standards against which you are going to compare the sample. Once again sourcing of suitable standards, their handling and storage, and preparation of the correct solution dilutions for standards are critical to the analysis.

13.     Quality control samples – CRM

Once the standards are run and system suitability established you finally get to inject your sample along with quality control materials or certified reference material which serves as a double check to ensure the accuracy of your results. These are certified samples having known composition and usually have an  acceptance criteria for the deviations permitted from the nominal value.

14.     HPLC software – control, run, acquire, process and report

To run the HPLC you need to set up a method on software for controlling the HPLC, acquire data from the system, define the sequence in which the samples and standard have to be injected, data processing parameters for integrating the acquired chromatograms and report the results.

15.     Preventive maintenance and trouble shooting

Things do not always go as planned and you might need to troubleshoot problems with the hardware or software or even with the sample preparation for that matter to be able to get correct results.

16.     Did the sample pass or fail?

After analysis you are required to communicate your verdict on the sample acceptability .Such a decision can have large implication in terms of production schedules and cost involvements. In legal matters the analysis report can be used as a legal document which can lead to prosecution. The reliability of your analysis is important and will depend on the precautions taken during the course of analysis such as use of validated methods, calibration of equipment and volumetric glassware and adherence to GLP guidelines.

17.     GLP, GDP and QMS – why do we need all these documents?

All requirements prescribed in the procedures such as GLP, GDP and QMS are to be complied with to report results which meet and satisfy your client. At first appearance the sheer size of documents and requirements is bound to scare you but if you adopt the practices in your work it will get streamlined and you will thank yourself for compliance with the established norms.

18.    Laboratory Information Management Systems (LIMS) – automate your laboratory operations

Use of sophisticated instrumental techniques improves quality of your results and increases your laboratory throughput but you can reap full benefit of automation by adopting laboratory information management systems which help manage your laboratory functions and also permit on – line sharing of results with your laboratory management and outside clients

Join the course now