Understanding the response factors of a GC detector

Gas Liquid Chromatography (GLC) or simply Gas chromatography (GC)is a versatile technique that helps identify and quantify constituent compounds of complex organic mixtures. Primarily such compounds should be volatile in nature and should not decompose at high temperatures inside the injector and the column oven.  The technique offers a wide choice of detectors. For any […]

10 Similarities between High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC)

The dissimilarities between HPLC and GC were discussed in the earlier article- How are Gas chromatography (GC) and High Performance liquid chromatography (HPLC) different?. The present article discusses 10 key features which are common to both the techniques. On going through these features you will recollect your basic understanding of chromatography and appreciate the commonality […]

How are Gas Chromatography (GC) and High Performance Liquid Chromatography(HPLC) different?

Gas Chromatography is an ideal technique for separation and identification of gases or volatile liquids having low molecular weights. The basis of separation is selective distribution of components present in gaseous phase on the solid adsorbent inside a column or on a thin liquid layer adsorbed on an inert solid support or on the inside wall […]

Advantages of Gas Chromatography (GC) over Thin Layer Chromatography (TLC)

Chromatography is a separation science which has made remarkable contributions to advances in fields such as pharmaceuticals, foods, nutraceuticals, environmental monitoring, forensics, cosmetics and life sciences. The potential for applications are enormous and new ones are being reported on an impressive scale. Chromatography evolved over the years from simple column chromatography to paper chromatography to […]

Importance of Gas leak detection before starting Gas Chromatographic Analysis

As a standard practice before starting GC operation gas leaks, if any, should be detected and rectified Why check for leaks? In any situation there is possibility of two types of gas leakages, namely, major leaks or minor leaks. Major leakages can result from cracked columns, damaged and loose fittings or ferrules or more commonly […]

Which type of gas regulator is suitable for Gas Chromatography?

Before answering the question you should understand the role a regulator plays. The regulator supplies gas at constant pressure to the Gas Chromatograph. Cylinder regulators are fixed direct to the gas cylinder valve and serve to reduce the cylinder pressure to a value suitable enough for the Gas Chromatograph. Such valves are of two types, […]

GC Free E-Course : Introduction to Gas Chromatography

Gas Chromatography is widely used for separation, identification and quantification of mixtures of liquids and gases. The majority of Gas Chromatography applications are found in Petroleum and Petro chemicals, Pharmaceuticals, Food & Flavors, Environmental and Forensics. The scope has widened through evolution of hyphenated techniques such as GC-MS, GC-FT-IR, GC-HS, etc which are capable of […]