HPLC or High Performance Liquid Chromatography has a marked presence in modern analytical laboratories. The major applications cover basic research and industrial quality control particularly in studies on biomolecules, pharmaceutical manufacturing and new drug development, stability studies on foods and pharmaceuticals and clinical monitoring of body fluids. The present article covers the contributions of HPLC […]
October 10, 2015 By 2 Comments
10 Similarities between High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC)
The dissimilarities between HPLC and GC were discussed in the earlier article- How are Gas chromatography (GC) and High Performance liquid chromatography (HPLC) different?. The present article discusses 10 key features which are common to both the techniques. On going through these features you will recollect your basic understanding of chromatography and appreciate the commonality […]
October 3, 2015 By 10 Comments
How are Gas Chromatography (GC) and High Performance Liquid Chromatography(HPLC) different?
Gas Chromatography is an ideal technique for separation and identification of gases or volatile liquids having low molecular weights. The basis of separation is selective distribution of components present in gaseous phase on the solid adsorbent inside a column or on a thin liquid layer adsorbed on an inert solid support or on the inside wall […]
May 9, 2014 By 2 Comments
How to Mix HPLC Mobile Phase Solvents for consistency of results?
A single solvent seldom meets the requirements for separations carried out using HPLC. In most cases two or more solvents are required to be mixed. In isocratic operation the composition is pre- decided and remains unchanged during the analytical run. On the other hand in gradient elution the final solvent composition is a lot different […]
April 17, 2014 By 2 Comments
What are the Benefits of UHPLC over normal Analytical HPLC?
The most desirable features of High Performance Liquid Chromatography a user looks forward to are: Highest resolution Greatest achievable sensitivity Fastest analysis speeds These became reality with the introduction of Ultra High Performance HPLC (UHPLC).Column efficiency was known to increase with reduction in particle size of stationary phase. Particle sizes in the range 3-5μm were used […]
April 12, 2014 By Leave a Comment
The role of Stationary Phase particle size on HPLC separations
Column length and particle size of stationary phase play a crucial role in separation efficiency and the column back pressure of HPLC columns. Longer columns and smaller particle size contribute to improved separation efficiency but at cost of increased column backpressure. Classification of HPLC separations by column dimensions covers the classification of HPLC separations into […]
April 2, 2014 By 23 Comments
What are the Differences between GC and HPLC?
Gas chromatography and High Performance Liquid Chromatography are both separation techniques which have gained immense popularity in both academic and industrial laboratories. High Performance Liquid Chromatography has found favour in applications covering pharmaceuticals, foods, life sciences and polymers whereas Gas Chromatography has significant applications in petroleum and petrochemical industry, flavours and fragrances and, environmental air monitoring,. A general […]
March 1, 2014 By 11 Comments
On-line Certificate Program on High Performance Liquid Chromatography – Join Now
Benefits : Learn what really goes into running a HPLC Participate in live webinar coaching sessions Test your pick up through quiz sessions Access to four important standard operating procedures More than 25 videos on operation, software and related laboratory practices Simplified approach calculations using Excel sheets Hard copy of certificate of participation Special discount […]
February 20, 2014 By 1 Comment
What are the Emerging Trends in HPLC Analysis?
Ever since its evolution High-Performance Liquid Chromatography has stood on a rock hard foundation and has seen several innovations which have met the growing expectations of chromatographers. Some of the desirable features and expectations of scientists are : High separation efficiencies with lowest column back pressures Reduced solvent consumption and high-speed analysis Low levels of […]
February 11, 2014 By Leave a Comment
How to ensure trouble-free HPLC System Operation
HPLC is the major support system in laboratories dealing with separation and identification of complex organic compounds. In industrial sector some laboratories are equipped with several HPLC systems in Quality control and R&D sections which run like workhorses 24 X7 hours for 365 days in a year. Downtime of these systems proves to be very expensive in terms […]
