Important suggestions for generating reliable HPLC results

HPLC has a marked presence in analytical research and quality control laboratories. Important decisions concerning both manufacturing processes as well as publication of research findings are based on decisions which can be arrived at only if high degree of reliability can be placed on the data generated by the HPLC system. HPLC is a multi […]

Contribution of HPLC to Nutritional Labeling of Foods

HPLC or High Performance Liquid Chromatography  has a   marked presence in modern analytical laboratories. The major applications cover basic research and industrial quality control particularly in studies on biomolecules, pharmaceutical manufacturing and new drug development, stability studies on foods and pharmaceuticals and clinical monitoring of body fluids. The present article covers the contributions of HPLC […]

10 Similarities between High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC)

The dissimilarities between HPLC and GC were discussed in the earlier article- How are Gas chromatography (GC) and High Performance liquid chromatography (HPLC) different?. The present article discusses 10 key features which are common to both the techniques. On going through these features you will recollect your basic understanding of chromatography and appreciate the commonality […]

How are Gas Chromatography (GC) and High Performance Liquid Chromatography(HPLC) different?

Gas Chromatography is an ideal technique for separation and identification of gases or volatile liquids having low molecular weights. The basis of separation is selective distribution of components present in gaseous phase on the solid adsorbent inside a column or on a thin liquid layer adsorbed on an inert solid support or on the inside wall […]

Advantages of Gas Chromatography (GC) over Thin Layer Chromatography (TLC)

Chromatography is a separation science which has made remarkable contributions to advances in fields such as pharmaceuticals, foods, nutraceuticals, environmental monitoring, forensics, cosmetics and life sciences. The potential for applications are enormous and new ones are being reported on an impressive scale. Chromatography evolved over the years from simple column chromatography to paper chromatography to […]

HPLC Quiz : Test Your Eligibility for Discount on Certificate Program on HPLC

A unique discount offer is being made to all those who are interested in joining the HPLC certificate program. The free HPLC e-course was developed to help you grasp the HPLC system component modules and upgrade your laboratory skills. We hope that you have benefited from the free e- course. Now it is our turn […]

How to Mix HPLC Mobile Phase Solvents for consistency of results?

A single solvent seldom meets the requirements for separations carried out using HPLC. In most cases two or more solvents are required to be mixed. In isocratic operation the composition is pre- decided and remains unchanged during the analytical run. On the other hand in gradient elution the final solvent composition is a lot different […]

What are the Benefits of UHPLC over normal Analytical HPLC?

The most desirable features of High Performance Liquid Chromatography  a user looks forward to are: Highest resolution Greatest achievable sensitivity Fastest analysis speeds These became reality with the introduction of Ultra High Performance HPLC (UHPLC).Column efficiency was known to increase with reduction in particle size of stationary phase. Particle sizes in the range 3-5μm were used […]

The role of Stationary Phase particle size on HPLC separations

Column length and particle size of stationary phase play a crucial role in separation efficiency and the column back pressure of HPLC columns. Longer columns and smaller particle size contribute to improved separation efficiency but at cost of increased column backpressure. Classification of HPLC separations by column dimensions covers the classification of HPLC separations into […]

On-line Certificate Program on High Performance Liquid Chromatography – Join Now

Benefits : Learn what really goes into running a HPLC Participate in live webinar coaching sessions Test your pick up through quiz sessions Access to four important standard operating procedures More than 25 videos on operation, software and related laboratory practices Simplified approach calculations using Excel sheets Hard copy of certificate of participation Special discount […]