Visualisation of Spots in Paper Chromatography

Coloured spots are easily observed on developed chromatograms. However, different approaches need to be adopted when colourless components are to be observed. It is convenient to classify such methods as specific or non-specific. Non-specific methods Iodine chamber – the developed plate is suspended in a closed jar containing a few crystals of iodine for about […]

Paper Chromatography : Sample Preparation and Application

Developing the Chromatogram involves three stages: Sample preparation Sample application Developing of the chromatogram Sample Preparation Samples can come either as liquids or solids. Liquids can be spotted without any pretreatment. However, solid samples need to be dissolved in a suitable solvent as only liquids can be applied to the chromatography paper. Spotting and Developing […]

Choice of Solvents for Paper Chromatography

A solvent plays an important role in resolution of sample constituents on the filter paper. For the purpose use can be made of a single pure solvent, undiluted solvent with water or a mixture of miscible solvents. The greater the distance between the resolved spots (measured by Rf values) better is the solvent mixture combination. […]

Types of Papers and their applications in Paper Chromatography

Paper chromatography makes use of paper which acts as a stationary phase. Paper essentially consists of cellulose fibers which are polymers having – OH functional groups sticking out of the polymer chains. These groups lead to retention and separation of surface absorbed molecules. In practice the separating molecules equilibrate between the layer of adsorbed water […]