Troubleshooting Tips for Gas Chromatographic Syringe

Gas chromatographic syringe is an indispensable accessory for the gas chromatographer. Syringe is necessary for introduction of selected volume into the gas chromatograph. The results of analysis are meaningful only if the selected volumes are accurately and reproducibly transferred into the system. Proper handling and care of chromatographic syringes has been covered earlier. However, in addition […]

Certificate Course on Gas Chromatography now available!

Finally the wait is over. Since the launch of our certificate courses on HPLC and Atomic Absorption Spectroscopy we have received several requests for a similar Course on Gas Chromatography. It has taken considerable time to develop the course as GC has wide-ranging applications in diverse areas and consolidation of details proved to be time-consuming. […]

Recommendations for switching from Gas cylinders to Laboratory gas generators

Industrial laboratories in sectors such as petroleum and petrochemicals, foods, flavours and fragrances and pharmaceuticals can house scores of gas chromatographs or their modified versions for specific applications. Such systems are bulk consumers of high purity gases such as nitrogen, helium, hydrogen and zero air 24 hours a day throughout the year. Even big R&D […]

What is Multi- dimensional GC?

Under normal GC operation a single column is mounted inside the column oven. This works fine with most common laboratory separations involving limited number of sample constituents (say,5 – 10). However, as the number of components having similar boiling points increases a single column is generally not sufficient to give the required resolution between the […]

How to prevent damage to Capillary GC columns

Capillary GC columns are capable of producing highly reproducible chromatograms provided right operational conditions are maintained and steps are taken from time to time to prevent damage and performance degradation. The factors which can result in deterioration are discussed in the present article and preventive measures are suggested: Accidental breakage High-temperature damage Oxygen damage Chemical […]

Benefits of Automated replacement of GC liners

The injector liners tend to get contaminated over a period of time due to injection of dirty samples. Due to adsorption on liner walls there is loss of some sample components and as a result there is a drop in sensitivity accompanied with broadening of the chromatographic peaks. The degree of deterioration of signals depends on […]

Types of Liners and their Selection

A GC inlet liner plays an important role in transfer of the injected sample into the GC column. It provides an inert surface for conversion of the injected liquid sample to vapour and transfer to the column without any change in its characteristics. Liners are available in different design configurations with or without deactivation and […]

Sample Injection Techniques for Capillary Column Gas Chromatography

Whenever you inject a sample into a Gas Chromatograph you are dealing with small micro- volumes of liquids which can be mixtures with differing volatilities in terms of their boiling points and other chemical characteristics. The desirable feature of the sample injection technique is reproducibility of injection volume injection after injection without loss of any […]

Minimization of influence of random fluctuations in operating conditions in GC analysis

More than often you set your operating conditions to your satisfaction but still face unexpected problems during analysis which cannot be reasoned and diagnosed easily. Such unexpected behaviour is quite frustrating and you have to spend considerable amount of your valuable time to iron out such abnormal variations in your analysis. In this article some […]

Why very long capillary GC columns are not preferred?

It is a well-known fact that the separation efficiency of a chromatographic column increases with an increase in its length. Doubling the column length increases resolution by around 40%. Normally capillary GC columns range in length from 15 m to about 60 m. Such column lengths are generally sufficient to resolve complex compounds having closely […]