Gas Chromatography

Gas Chromatography

Gas Chromatogram

 

Gas Chromatography is widely used for separation, identification and quantification of mixtures of liquids and gases. The majority of applications are found in Petroleum and Petro chemicals, Pharmaceuticals, Food & Flavors, Environmental and Forensics. The scope has widened through evolution of hyphenated techniques such as GC-MS, GC-FT-IR, GC-HS, etc which are capable of providing a vast expanse of information on the sample components.

Programme will be beneficial to

  • R&D Scientists
  • QA Managers & Practicing Chemists
  • Students in disciplines of Science Food &  Nutrition and Agricultural Sciences.
  • Petroleum & Petrochemical Scientists
  • Govt Regulatory Bodies
  • Environmental Scientists

Wide coverage based on GLP practices and our Standard operating procedures will be given to fundamental concepts of laboratory operations in a lucid manner so that both the novice and expert will find the programme  a useful reference for the day to day laboratory operations

Here are the links to the free online e-course on GC

Module 1 : Introduction to Gas Chromatography Course and its Objectives 

The overwhelming response to the HPLC free e-learning course has encouraged me to move ahead with the other courses announced earlier. It was a difficult choice to decide which course to launch next but based on opinion poll I decided to launch the GC programme and the next popular choice of free AAS course will follow soon thereafter. I understand that everyone has busy work schedules and today’s hectic life style leaves you little or no time to refer voluminous books to learn any technique…….

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Module 2 : Evolution of Gas Chromatography

Chromatography originated in early 1900 when Russian Botanist Mikhail S. Tswett separated plant pigments using calcium carbonate packed glass columns. It was not until mid century that the technique was applied to develop paper chromatography, High Performance Liquid Chromatography and Gas Chromatography…….

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Module 3 : Introduction to Gas Chromatography and Its Parts

The Gas Chromatograph appears to be a black box to the novice but an understanding of the components and their role will help raise your comfort level and help you generate data of highest reliability. You will also appreciate the role of preventive maintenance for long time usage and high reliability of results……. 

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Module 4 : Role of Gases in Gas Chromatography

“Formal education will make you a living, self education will make you a fortune “ — Jim Rohn

Gases play a crucial role in a Gas Chromatography system

• Transportation of injected sample to the column and subsequent transfer of separated components to the detector
• Act as fuel to support combustion in the detector…. 

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Module 5 : Types of Gas Chromatography Injectors

Sample injection in Gas Chromatography depends on the nature of sample. Sample volume should be kept minimal for best column efficiency. The sample should enter the column as a ‘plug’ of vapour to avoid brand broadening and loss of resolution…..  

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Module 6 : Types of Gas Chromatography Columns

In gas chromatography, the column is the heart of the system where the separation of sample components takes place. They are classified in terms of tubing dimensions and type of packing material. Packed columns are generally 1.5 – 10m in length and 2 – 4mm id. These are generally made of stainless steel or glass. On the other hand capillary columns are 0.1 – 0.5 mm id and can be 10 – 100m long….

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Module 7 : Types of Stationary Phases

“Optimism is the faith that leads to achievement, nothing can be done without hope and confidence” — Helen Keller

Most of the time a Gas Chromatographer is fortunate as he has to use a column specified for a particular method but there is a tendency to use the same column even though it is not the best choice for other samples. Apart from the basic column characteristics discussed in previous module stationary phase choice is a critical decision for achieving the right analytical conditions…. 

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Module 8 : Types of Gas Chromatography Detectors

The Gas Chromatography detector is capable of establishing both the identity and concentration of eluting components in the carrier gas stream. Before going further into the types of detectors it is essential to understand the nature of detectors and their desirable characteristics.

Non-selective
o Responds to all compounds present in carrier gas stream except the carrier gas itself…. 

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Module 9 : Gas Chromatography Applications

Gas Chromatography has been applied to identify and quantify the components of thousands of compounds present in complex matrices covering pharmaceuticals, foods & beverages, environmental samples, petroleum, polymers, forensic Science, etc. The application is further extended by choice of columns, system modules, detectors and use of hyphenated techniques such as GC – MS, GC – FT- IR, etc….. 

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Module 10 : Top 10 Interview Questions on Gas Chromatography

Your understanding of a topic and technical skills are gauged in interviews. We now provide you an opportunity to familiarize yourself with some typical questions that you may face in interviews when seeking career opportunities in analytical laboratories involving extensive use of gas chromatography technique…. 

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