Understanding the Linearity of a Calibration Plot

The essence of a calibration plot is the linear relation between two variables. How do you decide the degree of linearity to rely on interpretations based on the calibration plot. The points on a calibration line will seldom fall on a perfect straight line. Normally you would use your ruler to draw a straight line […]

Guidelines on Generation and Interpretation of Calibration Plots

Your choice of an analytical technique for a particular analysis would be based on its applicability and a conviction that the response will be linear to changes in concentration. The graphical representation of concentration as x-axis and corresponding instrument response on y- axis is referred to as a calibration plot. The concentration of unknown is […]

Concentration Calculations in Analysis – A Primer

Most of us were exposed to chemical calculations in school and college days. As we pursued higher degrees and progressed in our career as analytical scientists we took more interest in learning new techniques and picking up operational details of analytical instruments. It cannot be denied that our pursuit provided us greater self confidence of […]

Measurement Units Commonly Used in Analytical Work

As an analytical scientist your main concern would have been to report your findings in terms of what is present in your sample and in what amount. Most instruments convert input in other units to concentration or other required units. You might be inclined to think that it is sufficient to have a knowledge of […]

How to make a calibration curve and calculate sample concentrations using Excel – Video Tutorial

Excel Calibration Curve Video TutorialWorking in the laboratory, there are a number of different ways that we can calculate the amount of an analyte present in a sample by comparing them to standards. You could use a single external standard, a calibration curve,  internal standard or use standard addition. Learning to use these different methods […]