What are the Differences between GC and HPLC?

Gas chromatography and High Performance Liquid Chromatography are both separation techniques which have gained immense popularity in both academic and industrial laboratories. High Performance Liquid Chromatography has found favour in applications covering pharmaceuticals, foods, life sciences and polymers whereas Gas Chromatography has significant applications in petroleum and petrochemical industry, flavours and fragrances and, environmental air monitoring,. A general […]

How to Read a Chromatogram?

Over the years chromatography has gained an enviable position in analytical laboratories involving separation and quantification of organic compound mixtures. However, a chromatogram is not a display of results in concentration units but rather a graphical display in real time of peaks generated as the separated components pass through the detector. The chromatogram makes little […]

Proper Use, Handling and Care of Chromatographic Syringes

A syringe is a high precision liquid measurement and transfer device capable of maintaining its designed performance if proper care is exercised in its use, handling and storage. Proper use Physically examine for cracks, bent needles or dried residues before putting syringe to use Use syringe for dispensing liquids at room temperature only Rinse the […]

Understanding Chromatographic Syringe Nomenclature

Most of us have used a syringe for introduction of samples into a chromatograph numerous times without bothering to understand its terminology and specifications. Such features specify its component parts and helps in making a correct choice for the desired application. Let us familiarize ourselves with the nomenclature and specifications of syringes used in chromatography […]

What is Chromatography?

As per dictionary definition Chromatography is a method for separating the constituents of a solution(gas or liquid) by exploiting the different properties of different molecules. The technique employs a mobile phase(gas or liquid)to transport the solution to be analyzed through the stationary phase (solid or liquid)which absorbs or impedes different components of the solution to […]

What is HPLC?

The basic question that needs a simple answer is “What is HPLC?” In simple terms HPLC is an analytical technique used for separation of components of organic mixture of compounds when such compounds are nonvolatile, thermally unstable and have relatively high molecular weights. A liquid carrier stream termed as the mobile phase serves to carry […]

How Desirable Detector Characteristics Influence your Chromatographic separations?

Role of a Detector A detector is the final component in the chromatographic system and provides real time information on the separated components. After separation of the components by the chromatographic column they move sequentially to the detector and generate response in proportion to the concentration of solute in the mobile phase stream. Chromatographic detection […]

How to calculate System Suitability in Chromatography

In my earlier post on generation of authentic chromatographic data I had emphasized the need for evaluation of system suitability before proceeding with analysis. Some factors contributing to system suitability failures in HPLC were discussed. The current post introduces you to system suitability parameters and their acceptance limits. Resolution Resolution is a measure of the […]

How do I generate authentic Chromatographic results?

As an analytical chemist your primary concern is to generate authentic data which is universally accepted and reproducible under identical conditions in any other laboratory. Even the use of most sophisticated instruments will not serve the purpose if globally accepted norms are overlooked. System suitability is the key to generation of valid data in chemical […]