What is Chromatogram & How to Read a Chromatogram?
Over the years chromatography has gained an enviable position in analytical laboratories. You have been introduced to simple concepts on how to read a chromatogram.
Browse all Chromatography articles, guides, and protocols.
Over the years chromatography has gained an enviable position in analytical laboratories. You have been introduced to simple concepts on how to read a chromatogram.
GC and HPLC are both separation techniques which have gained immense popularity in both academic and industrial laboratories.
Chromatographic Syringe for introduction of samples into a chromatograph numerous times without bothering to understand its terminology and specifications
Chromatography is an invaluable analytical technique in the hands of the analytical scientist for the separation and quantification
What is HPLC? In simple terms HPLC (High Performance Liquid Chromatography) is an analytical technique used for separation of components of organic mixture of compounds.
A detector is the final component in the chromatographic system and provides real time information on the separated components, the detector characteristics influence the separation.
In my earlier post on generation of authentic chromatographic data I had emphasized the need for evaluation of system suitability before proceeding with analysis. Some factors contributing to system suitability failures in HPLC were discussed. The current post introduces you to system suitability parameters and their acceptance limits.
System suitability must be performed before and throughout all regulated assays. It is not sufficient to apply suitability tests at the beginning of the chromatographic run and assume that the system will perform properly during the run. Test mixtures containing main components and expected impurities can be injected at the beginning and interspersed during testing.
Paper Chromatography is one of the earliest and simplest chromatographic techniques alongwith other advanced versions such as ✔TLC ✔HPLC ✔GC and hyphenated techniques.
Partition chromatography definition states that it is a technique mainly used for the separation of the components present in the mixture into two liquid phases
A Chromatographic Syringes is a high precision liquid measurement and transfer device capable of maintaining its designed performance