Key Considerations in preparation and use of HPLC Mobile Phase

Preparing and using an HPLC mobile phase means selecting and combining component solvents whose properties are compatible with both the sample and the column packing material, since the reliability of an HPLC analysis depends heavily on these precautions.

The reliability of any HPLC analysis depends to a great extent on precautions and considerations taken on nature of the sample and characteristic properties of component solvents of the mobile phase.In other words the mobile phase is the lifeline of HPLC system and its compatibility with the sample and column packing material is a major consideration before taking up any analysis.