Reverse Phase Chromatography
Reverse phase chromatography is established on the partition equilibrium between the non-polar (hydrophobic) stationary phase and the polar mobile phase.
HPLC
Browse all HPLC articles, guides, and protocols.
Benefits of Automated injections in chromatographic analysis
Automated injection via a programmable autosampler improves the accuracy, consistency, and throughput of chromatographic results over manual injection methods.
Suggestions for trouble free operation of your HPLC system
Practical tips for trouble-free HPLC operation: system inspection, mobile phase filtering, air bubble removal, injector care, and start-up/shutdown steps.
Importance of HPLC grade solvents in HPLC analysis
HPLC grade solvents are distilled and filtered to over 99.9 percent purity, and their UV cut-off, viscosity, and stability directly affect result reliability.
Guidelines on use of buffers in HPLC separations
Buffers in HPLC separations control ionization of acidic or basic samples, requiring careful selection of pKa, pH range, and concentration for reproducible results.
HPLC - The new age analytical technique!
The flexibility of HPLC in sample scale, stationary phases, detectors, and mobile phases makes it dominant in pharma, food, and environmental analysis.
Critical considerations in HPLC solvent selection
Selecting HPLC mobile phase solvents means weighing cost, solubility, UV absorbance, volatility, viscosity, and inertness to avoid degrading separation performance.
Important role of preventive maintenance in smooth functioning of HPLC systems
Preventive maintenance for HPLC systems covers regular upkeep of the mobile phase, columns, pumps, injectors, and detectors to avoid breakdowns.
How are analytical scale and preparative scale HPLC different?
HPLC is a versatile analysis tool that affords separation and quantification of large range of chemical compounds.
Which technique to use – GC or HPLC?
The focus of the present article is on differences between GC and HPLC techniques so as to help you decide the appropriate technique for the particular analysis in hand.
Role of HPLC in Cleaning Validation of Laboratory Glassware
How HPLC with UV or photodiode array detection is used to validate that laboratory glassware is free of residues before being reused for sensitive analysis.
Restoration of HPLC Column Performance
Restoring HPLC column performance reverses the peak shape and resolution decline columns develop over time, using a flushing sequence matched to the stationary phase.
Important suggestions for generating reliable HPLC results
Reliable HPLC results depend on properly maintaining every instrument component, including the mobile phase, sample, column, pump, injector, and detector.
Time saving tips for the HPLC user
Practical tips for HPLC laboratories to save time and increase throughput through staff training, advance work planning, and preventive instrument maintenance.
How to minimize cost on HPLC column replacements?
Minimizing HPLC column replacement costs means extending column life through careful handling and storage, since columns are a major recurring cost in HPLC analysis.
Advantages of Filtering HPLC mobile phase solvents
Filtering HPLC mobile phase solvents removes suspended particulates that can build up in columns, raise back pressure, and damage pump components over time.
Potential applications of HPLC in Clinical laboratories
The article discusses briefly about some common HPLC analysis applications in clinical laboratories.
Contribution of HPLC to Nutritional Labeling of Foods
HPLC is a useful technique for analysis of nutrition content of foods
Advantages of HPLC over Thin-layer Chromatography (TLC)
A comparison on characteristic features of HPLC that make it score over TLC. Click here to read more about the advantages of HPLC over Thin-layer Chromatography.
What are guard columns and what benefits they offer in HPLC analysis?
The efficient operation of HPLC system is dependent on freedom of mobile phase and sample from chemical impurities or solid suspensions.
Solutions to First HPLC Program Quiz
The first HPLC quiz was published on the site on 18 June 2014 and I am glad to inform that a number of our subscribers scored over 80% in response to the discount offer and have earned 20% discount on the course fee.
HPLC
HPLC is a separation technique which is extensively used to separate, identify and quantify components of mixtures of organic compounds selectively
HPLC – which modules require regular calibration?
HPLC is a popular analysis technique and several reputed makes are available. On first appearance it looks like a modular boxes and an intricate bunch of tubings
Essential precautions in handling and use of HPLC mobile phase
Handling the HPLC mobile phase properly covers solvent quality, consistent mixing, filtration, degassing, and storage to ensure repeatable chromatographic results.