HPLC stands for High Performance Liquid Chromatography.

Its earlier name was High Pressure Liquid Chromatography because it involved use of liquid mobile phase requiring higher pressures than gases used in Gas Chromatography. The technique has found immense scope of applications in both academic and industrial laboratories requiring identification and quantification of mixtures of organic compounds.

Benefits of Automated injections in chromatographic analysis

The accuracy and precision of chromatographic results depend to a large extent on the repeatability of precise injection volumes into the chromatographic systems. Such volumes generally fall in µl range. Other contributing factors responsible for the consistency of results are operational parameters such as mobile phase composition and flow rate, control of the temperature of […]

Suggestions for trouble free operation of your HPLC system

Regular maintenance is strongly recommended for a smooth and trouble-free operation of all analytical instruments. In addition, proper handling and operation will also contribute to long service life and quality of data obtained from your instruments. The recommendation is even more significant for HPLC as it is one instrument which works like a workhorse round […]

Importance of HPLC grade solvents in HPLC analysis

HPLC has established itself as a versatile analysis technique in research, industrial and commercial testing laboratories. The area of applications is increasing by the day as is evidenced by journals and publications on the subject. However, high reliability on results can be placed only if high purity grade materials are used in addition to adherence to […]

Guidelines on use of buffers in HPLC separations

Buffers play an important role in Reverse phase HPLC separations. Changes in pH influence the degree of ionization of sample molecules. The change is minimal in case of non-polar molecules but assumes significance in case of polar molecules which are acidic or basic in nature. Changing the pH can be used effectively to separate closely spaced […]

HPLC – The new age analytical technique!

Analytical chemistry is becoming a popular choice as a career option for students pursuing the discipline of chemistry. The obvious reason is that it applies knowledge of chemistry to gain understanding of chemical properties of materials. It makes valuable contribution in establishing the quality of natural resources and synthetic industrial products for our consumption. The […]

Critical considerations in HPLC solvent selection

Selection of solvents for use as a mobile phase in HPLC analysis is a key component of method development. It is not possible to hava a universal solvent which will meet all applications and more than often a combination of solvents is decided based on the analysis requirements. Selection of suitable solvents is based on […]

Important role of preventive maintenance in smooth functioning of HPLC systems

Preventive maintenance plays a key role in trouble-free operation of laboratory instruments. It is even more critical for instruments that are required to operate round the clock. HPLC is one such instrument which is expected to run continuously in commercial testing laboratories and industries. In pharma and food manufacturing industries quality of products is required […]

How are analytical scale and preparative scale HPLC different?

HPLC is a versatile analysis tool that affords separation and quantification of large range of chemical compounds. It finds unlimited applications in basic research, pharmaceuticals, foods, polymers and other similar applications. The availability of numerous columns, stationary phases, mobile phases and detectors makes HPLC a high utility facility in modern laboratories. Analytical scale HPLC is […]

Which technique to use – GC or HPLC?

Chromatography has been used as a versatile technique in the laboratory for separation, identification and quantification of components in mixtures of organic compounds. In the earlier stages Chromatography evolved from column chromatography and planar chromatographic separations based on paper chromatography and thin layer chromatography. Advances in instrumentation technology led to introduction of Gas Chromatography(GC) and High […]

Role of HPLC in Cleaning Validation of Laboratory Glassware

HPLC has made significant contributions in detection and quantitative estimation of organic compounds. In pharmaceutical analysis HPLC has been extensively used in assay of the active ingredient as well as related impurities which are present in formulations in very low concentrations. Estimation of related substances was not possible using other conventional analysis techniques. This unique […]