What Are the Desirable Properties of HPLC Mobile Phase?
Mobile phase has been rightly termed as the lifeline of the HPLC system. It plays the important role of transport of the sample through the separation column.
HPLC
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HPLC Quiz : Test Your Eligibility for Discount on Certificate Program on HPLC
The free HPLC e-course was developed to help you grasp the HPLC system component modules and upgrade your laboratory skills. Now here is this quiz to test your skills
How to Mix HPLC Mobile Phase Solvents for consistency of results?
A single solvent seldom meets the requirements for separations carried out using HPLC. In most cases two or more solvents are required to be mixed.
What are the Benefits of UHPLC over normal Analytical HPLC?
HPLC is an expensive technique involving use of high purity grade solvents. Adopt UHPLC to get the best out of your High Performance Liquid Chromatography analysis
The role of Stationary Phase particle size on HPLC separations
Column length and particle size of stationary phase play a crucial role in separation efficiency and the column back pressure of HPLC columns.
USP Classification of HPLC Columns
All major HPLC manufacturers provide HPLC columns to meet different analytical requirements under their trade names.
What are the Emerging Trends in HPLC Analysis?
Ever since its evolution HPLC has stood on a rock hard foundation and has seen several innovations which have met the growing expectations of chromatographers.
How to ensure trouble-free HPLC System Operation
HPLC is the major support system in laboratories dealing with separation and identification of complex organic compounds.
HPLC Free e-course
HPLC is applied to separation and quantitation of components of mixture of compounds that may not be stable at higher temperatures encountered in Gas chromatographic separations.
Cost Saving Tips for the HPLC User
HPLC analysis involves use of expensive mobile phase solvents and recurring costs on consumables such as HPLC columns, sample and solvent filters, vials, glassware, etc.
How to Deprotenize Biological Fluids prior to HPLC analysis?
Deproteinization has contributed to the ever expanding scope of HPLC analysis of biological fluids. Sample filtration before HPLC analysis.
Common Peak Shape Distortions in HPLC and their Prevention
A chromatographer always looks forward to getting perfect shaped peaks for each and every analysis but in reality peaks get distorted due to numerous reasons.
Prevention of Retention Time Drifts in HPLC Analysis
Retention time of a peak characterizes the identity of the eluting compound. Retention time has been explained in the earlier article How to read a chromatogram?.
Advantages and Disadvantages of different HPLC Pump Configurations
A brief introduction to different types of HPLC pumps was provided in module 7 of our free course on HPLC.
Advances in HPLC Stationary phases in Zirconia HPLC columns
Silica-based HPLC columns have remained workhorses in HPLC laboratories. Over the years with advances in particle technologies size reduction has been possible from 5μm to sub- 2μm which led to development of UHPLC.
Ion pair chromatography in reverse phase HPLC
Ion pair chromatography helps separate charged analytes using the popular reverse phase HPLC.
Classification of HPLC Separations by Column Dimensions
Based on the available information on the sample and purpose of analysis HPLC methods are classified into the following four basic modes
Core Shell Column Packings- A Challenge to UHPLC
The era of UHPLC or fast LC started in 2004 when sub 2 – μm particles were introduced. Typically modern ultrafast UHPLC columns are 15 mm long with an internal diameter of 2.1 mm in comparison to typical HPLC columns which are 150 – 250 mm long with 4.6 mm id.
Care of HPLC Columns
A HPLC column is the most critical component of the HPLC system. Quality of your results will greatly depend on its performance.
On-line Mixing of HPLC Solvents
HPLC analysis commonly uses Isocratic or Gradient elution. In Isocratic mode the mobile phase composition remains unchanged during the analysis run whereas in gradient elution the mobile phase composition changes as per defined program during the run.
Considerations for Mixing of HPLC Mobile Phase Solvents
It will be very rare that you will come across an HPLC method which uses a single solvent throughout the run. Pure solvents kept in mobile phase reservoirs need to be mixed in required proportions under isocratic conditions.
10 Useful tips on purchasing HPLC
HPLC technique is fast emerging as a popular analytical technique and the system is finding a prominent place in most industrial and academic research labs.
HPLC certificate program released!!!
In addition to the in depth coverage there are over 25 videos that depict software control of instrument and other associated HPLC laboratory operations.
What really goes into running a HPLC: Infographic
We answer the question with a 18 step guide on running a HPLC presented as a lively Infographic, free to download and share with your friends and team mates!