Understanding the response factors of a GC detector

Gas Liquid Chromatography (GLC) or simply Gas chromatography (GC)is a versatile technique that helps identify and quantify constituent compounds of complex organic mixtures. Primarily such compounds should be volatile in nature and should not decompose at high temperatures inside the injector and the column oven.  The technique offers a wide choice of detectors. For any […]

Selecting a suitable detector for Gas Chromatographic analysis

Gas Chromatography offers a wide range of detectors to meet your analysis applications. The two detectors which are in common use are the Flame Ionisation detector and Thermal Conductivity detector as these cover detection of majority of compounds encountered in routine applications. However, there are other detectors also available which are useful for specific applications. […]

Influence of changes in operational conditions on Gas Chromatograms

Gas Chromatography contributes significantly in testing and quality control laboratories in petrochemicals and fuels, foods, pharmaceuticals, flavours and fragrances and cosmetics industries. The technique offers high sensitivity and even minor changes in operational conditions have a marked bearing on chromatographic outputs. Such changes can lead to misleading interpretations and conclusions based on observations. The article […]

Improve reproducibility of syringe injections for GC analysis

Reliable quantitative analysis requires high degree of reproducibility of sample volume injections. Auto samplers provide the desired reproducibility of injection volumes and in addition automate the analysis sequence thereby saving your precious time. However, even to this day, syringe manual injections are the most widely used sample injection technique. Syringe injection requires development of right […]

Simple steps to minimize Column bleed from GC columns

Reduction of column bleed requires first of all a clear understanding of the term. Column bleeding results from thermal breakdown of the stationary phase at temperatures close to the upper temperature limit of the column. The level of bleed depends on the film thickness of the stationary phase layer on the adsorbent. Thick films columns […]

Sampling of Gases for analysis by Gas Chromatography

Gas chromatography is an ideal tool for analysis of gases. The versatility of the technique extends its capability to analyse samples such as dissolved solids, liquids and gases. The scope of applications extends from pharmaceuticals, foods, environmental monitoring, petroleum refining, and workplace environment monitoring for safety of workers. Gas samples require special handling as gases […]

Benefits offered by automated injection in Gas Chromatography

Manual syringe injection is the most popular gas chromatography sample injection practice adopted in laboratories across the world. The obvious reason for this choice is convenience and low price of syringes. However, keep in mind that it may take considerable time to perfect the skills required to inject volumes with high accuracy and precision. Bear […]

How to minimize retention time drifts in Gas Chromatography?

Retention time drifts contribute to wastage of time and undue frustration during analytical chromatographic runs. It is defined as the time that the particular compound spends in the column between its injection and detection. In other words it is the time that elapses between its injection and the maximum of its peak response. It helps […]

Familiarize with the Gas Chromatograph

A Gas Chromatograph like any other analytical instrument has evolved from one with several knobs and dials to one having a simple microprocessor based keypad to control the operational parameters. The simplification has resulted in ease of operation and time saving. An understanding of the main component parts will help in maximum utilization of system capabilities.

Tips on improving accuracy and precision of Gas Chromatographic injections

The accuracy and precision of Gas Chromatographic analysis depends to a significant extent on your skill to use the injection syringe. It is possible to achieve a precision of manual injection volumes less than 1% RSD once you have gained mastery on sample injections. Proper handling and care of chromatographic syringes has been dealt with earlier. […]