10 Similarities Between HPLC and GC (Gas Chromatography)
The present article discusses 10 key features which are common to HPLC and GC techniques. These features will recollect your basic understanding of chromatography.
Gas Chromatography
Browse all Gas Chromatography articles, guides, and protocols.
How are Gas Chromatography (GC) and High Performance Liquid Chromatography(HPLC) different?
HPLC and GC are established techniques used in academic and industrial laboratories. A thorough understanding of their differences is helpful in making the right choice for your sample analysis.
Advantages of Gas Chromatography (GC) over Thin Layer Chromatography (TLC)
Gas Chromatography has a distinct edge over Thin Layer Chromatography .It affords highest resolution and sensitivity for complex mixtures through choice from a vast range of columns, detectors and control over operational parameters.
Gas Liquid Chromatography
The distinction between Gas Solid chromatography (GSC) and Gas Liquid chromatography (GLC) is often not clearly understood.
GC Column
GC column is a delicate item which can retain its operational characteristics over an extended period provided its handling and operational guidelines are diligently followed.
Now GC Method Optimization and Development on your fingertips!
As a research chromatographer you are required to develop a method or optimize an existing application.
Temperature Control of the Gas Chromatographic Column
The Gas Chromatographic column can either be maintained at a uniform temperature during the analysis or its temperature can be varied at a predetermined rate.
Factors Governing the Resolution of peaks in the Gas
The operational parameters can be optimized to your advantage to get the best output from your gas chromatograph.
How to save your time by preventing Gas leaks before running the Gas Chromatograph?
Leaks should be checked and eliminated before proceeding for column conditioning and start of analytical work on the Gas chromatograph.
Importance of Gas leak detection before starting Gas Chromatographic Analysis
In essence you can achieve a high degree of GC performance and reproducibility of results once you ensure freedom from leaks before running the GC system.
Gas Chromatography in Petroleum Refining Industry
Gas chromatography has found large-scale applications in monitoring of petroleum refining and finished product testing. Click here to read more.
Chromatographic Techniques for Analysis of Flavours in Foods
Gas chromatography alone or in combination with headspace analysis or mass selective detection has found widespread application and analysis of flavours in foods and beverages.
GC
Follow the suggested steps to prolong the resolving power of your caplliary GC column
Which type of gas regulator is suitable for Gas Chromatography?
The regulator supplies gas at constant pressure to the Gas Chromatography. Know your requirements before selection of the gas regulator.
Why Capillary Columns Are Preferred over Packed Columns in GC
Why capillary columns are better than packed ones, let’s learn about each of them individually. This will help you understand the differences in a better way.
Hydrogen, Helium or Nitrogen: Which Is the Most Suitable as a Carrier Gas?
A carrier gas serves as a medium for transport of eluting molecules through the Chromatograph in Gas Chromatography.
How to handle Gas Chromatographic Gases Safely?
The Gas Chromatographic gases are supplied in compressed gas cylinders which are colour coded and require careful handling to prevent accidents.
Why is Leak checking necessary before starting Gas Chromatography analysis?
Remember to turn off the oven fan when checking Gas Chromatography column leaks.
Split or Splitless Injection in Capillary Gas Chromatography
Benefits of split/splitless injection in Gas Chromatography detection gives you the freedom of changing the amount of sample component entering the capillary column.
Why is Conditioning necessary for GC Columns?
Conditioning is a process involving flow of carrier gas through a GC column at elevated temperature to flush out residual contaminants and make it fit for reliable use.
Four Key Considerations for choice of Capillary Gas Chromatography Columns
Choice of Gas Chromatography column internal diameter depends on the concentration of the sample. When sample loading exceeds Gas Chromatography column capacity there is loss of resolution, poor reproducibility and peak distortion.
Gas Purification Requirements in Gas Chromatography
Gas purification of commercially available gas supplies is necessary to generate stable baselines without noise or ghost peaks in gas chromatographic analysis.
Sample Injection Syringe in Gas Chromatography
Sample injection is a critical part of Gas Chromatography analysis. A number of errors do creep in if due consideration is not given to the proper choice of sample introduction syringe.
Why are HPLC columns shorter than GC columns?
HPLC and GC are separation techniques which have gained a strong foothold in chemical laboratories.