What is Stray light and how it is monitored?
Stray light is any light reaching the detector that lies outside the wavelength band width selected for analysis by the monochromator.
Spectroscopy
Browse all Spectroscopy articles, guides, and protocols.
Factors governing choice of Light Sources for UV-VIS Spectrophotometers
Unfortunately there is no single source that is suitable over the entire UV– VIS spectral region (190 -800 nm).
How to ensure accuracy in Quantitative estimations - UV – VIS
UV – VIS spectroscopy offers a fast and accurate estimation of analytes in diverse range of applications. The sensitivity of the technique is further enhanced by reaction with reagents that impart colour to otherwise colourless species.
What is the basis of colour of substances?
Colours all around us do fascinate us but have you ever wondered that even though the natural light is colourless then what it is that imparts different colours to different materials.
UV – VIS Absorption Spectrum
An understanding of the UV – VIS absorption spectrum is important for full exploitation of the potential of your spectrophotometer.. UV- VIS spectroscopy is not only the oldest spectroscopic technique but is also a forefather of instrumental methods of analysis.
spectroscopic analysis
A light source is an essential component of a spectrometer as it provides the required energy for excitation essential for spectral transitions.
What Physical Changes Take Place When a Light Beam Passes through a Different Optical Media?
This article discusses about the interactions when a light beam passes through a different optical media. Read more.
Useful Tips to Ensure Top Performance of Your FT- IR Spectrometer
FT-IR systems are sensitive to increased humidity as the optical windows are made of hygroscopic materials. These tips will help in increasing their lifetime.
Scope of FT IR Spectroscopic applications in Near and Far IR Regions
Within the IR range mid-IR region is of special interest and an earlier article on mid-IR applications has touched upon typical applications in this area. On the other hand contributions of near- IR and far- IR cannot be overlooked and deserve a mention.
FT -IR Spectroscopy
FT -IR Spectroscopy has widespread applications in molecular structural confirmation and characterization of solids, liquids and gases.
ibre Optic Probes – Extension Arms of Mid – IR Spectroscopy
IR spectroscopy and in particular mid-IR region provides a wealth of information on chemical composition of materials. Sampling techniques and HATR accessory in particular have contributed immensely to the scope of applications.
HATR – a versatile FT–IR Sampling Accessory
HATR or Horizontal Attenuated Total Reflectance accessory offers a number of benefits over conventional sampling methods adopted for liquids and solids.
Comparison of different Solid Sampling Techniques for FT- IR Spectroscopy
Apart from liquids solids constitute a major segment of samples characterized using FT-IR spectroscopy. The merits and de-merits of each technique are discussed in this article.
Merits and De-merits of different Liquid Sampling accessories used in FT-IR Spectroscopy
Liquid samples constitute a large majority of samples requiring identification and characterization using FT-IR Spectroscopy
What Are Chromophores and Chromophoric Shifts? | Lab Training
Have you ever wondered why some compounds are colourless whereas some are coloured. Read more about chromophores and chromophoric shifts.
FT-IR Spectroscopy
FT-IR Spectroscopy has made significant contributions in identification and characterization of materials. The origins of infrared spectroscopy as an analytical tool can be traced back to the pioneering work of Sir William Herschel in 1800.
Classification of FT-IR Spectroscopy Detectors
High-speed scanning applications requiring higher sensitivities such as hyphenated techniques particularly FT-IR coupled with GC give excellent sensitivity and speed of scanning.
IR spectroscopic analysis
Glass is a common window material in the visible region because of its transparency. However, it is not suitable for IR region because it strongly absorbs IR radiation. Quartz which is suitable in UV region is not of much use in IR studies
Contributions of Michelson Interferometer to FT-IR Spectroscopy
The reason for this has been the several advantages offered by FT-IR spectroscopy technique.
Applications of IR Spectroscopy – Mid-IR Region
IR spectroscopy is a popular qualitative identification technique which uses comparison of the spectra of unknown sample with the spectra of known standard materials. Such spectra can be manually overlaid for peak to peak comparisons
Applications of UV – VIS Spectroscopy
UV – VIS Spectroscopy exploits the absorption of light in the UV and visible regions of the electromagnetic spectrum by chemical absorbing species.
Applications of Spectroscopic analysis
Spectroscopy is one of the earliest instrumental methods of analysis which has made in-roads into almost every sphere of basic and applied research as well as manufacturing of consumer goods.
Proper Care and Handling of UV-VIS Absorption Measurement Cells
UV – VIS absorption measurement cells or cuvettes as they are commonly called are glass or quartz rectangular cells which are used for holding the sample and reference solutions in beam path for making absorbance measurements.
Parameters influence Spectral absorbance
It is well established that instrument parameters and settings influence the absorbance measurements and appearance of absorbance spectra.